Fig. 2
From: Targeting Akt by SC66 triggers GSK-3β mediated apoptosis in colon cancer therapy
SC66 triggered cell apoptosis in a p53-independent pathway. a Cell apoptosis was analyzed by fluorescence-activated cell sorting (FACS) analysis after HCT-116 WT, HCT-116 p53−/− and DLD1 cells were treated with 2 μg/ml SC66 for 24 h. The percentage of apoptotic cells were calculated from fluorescence activated cell sorting (FACS) analysis. Data represent the mean ± SD of three independent experiments. ***P < 0.001 vs untreated control cells. b Hoechst 33342 morphological examination of apoptosis in HCT-116 WT, HCT-116 p53−/− and DLD1 cells were treated with 2 μg/ml SC66 and incubated for 24 h, then stained with Hoechst 33342. c Bax multimerization in mitochondria fraction was detected by Western blotting after DSP cross-link. d Western blotting show the expression of p53, PUMA, Bax6A7 and cleaved Caspase 3 in HCT-116 with 2 μg/ml SC66 for 24 h. Similar results were obtained from three independent experiments