Skip to main content
Fig. 4 | Cancer Cell International

Fig. 4

From: Targeting Akt by SC66 triggers GSK-3β mediated apoptosis in colon cancer therapy

Fig. 4

GSK-3β activated Bax directly and inhibited Bcl-xL indirectly, leading to SC66-induced apoptosis. a, b The interaction between GSK-3β and Bax increased after SC66 treatment. Co-immunoprecipitation with an anti-GSK-3β antibody or anti-Bax antibody was used to pull down GSK-3β or Bax. a Western blotting for Bax showed the amount of Bax binding to GSK-3β. b Western blotting for GSK-3β showed the amount of GSK-3β binding to Bax. IgG was used to as a control for the GSK-3β-specific antibody. c, d The effect of GSK-3β knockdown or GSK-3β over-expression on colon cancer cells. c Bax6A7, Bcl-xL and GSK-3β were detected in HCT-116 WT following the treatment of 2 μg/ml SC66, with GSK-3β knockdown or not. Similar results were obtained from three independent experiments. d HCT116 WT were transfected with either empty vector or a constitutively-active GSK-3β expression constructs for 24 h, following 24 h of 2 μg/ml SC66 treatment. The levels of Bax6A7, Bcl-xL and GSK-3β were analyzed by Western blotting. Similar results were obtained from three independent experiments

Back to article page