Fig. 5
From: Knockdown of PKM2 enhances radiosensitivity of cervical cancer cells
Western blot for the detection of stemness-associated markers. a Cells were pretreated with or without 2 Gy of IR and were analyzed for the expression of cleaved caspase 3, cleaved caspase 9, caspase 3, caspase 9, Bax, and Bcl2 protein levels through Western blotting. b Knockdown of PKM2 combined with IR downregulated NANOG/OCT4/SOX2 in protein expression. GAPDH protein was used as an internal standard. Data represent three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. (shPKM2: shRNA against PKM2; IR: irradiation. 1 = control cells, 2 = control cells + IR, 3 = shPKM2 cells, 4 = shPKM2 cells + IR)