Fig. 2

BMX-ARHGAP upregulation induces SGC7901 stem cell formation, proliferation and invasion. a Protein expression of CD133, CD44, SOX2 and Nanog in transfected SGC7901 cells determined by western blot assay; b CD133⁺CD44⁺ cells sorted by flow cytometry; c the number of formed cell spheres after transfection shown by cell sphere formation assay ( ×200); d the number of formed cell colonies after transfection shown by cell colony formation assay; e, the invasion ability of CD133⁺ and CD44⁺ evaluated by Transwell assay (×200); f proliferation ability of CD133⁺ and CD44⁺ evaluated by EdU labeling assay (×100). *p < 0.05 vs. the pcDNA3.1-transfected cells. Measurement data were expressed as mean ± standard deviation and analyzed by independent t-test; each experiment was repeated three times. BMX bone marrow kinase on chromosome X, ARHGAP RhoA GTPase activating protein