Fig. 5

BMX-ARHGAP overexpression enhances tumorigenicity of SGC7901 cells in nude mice. a Tumor volume and weight in nude mice injected with transfected SGC7901 cells; b protein expression of CD133, CD44, SOX2 and Nanog in the tumor tissues of nude mice injected with transfected GC cells, as determined by western blot assay; c protein expression of SOX2 and Nanog together with the extents of JAK2 and STAT3 phosphorylation in the tumor tissues of nude mice injected with transfected SGC7901 cells, as measured by immunohistochemical staining (×200); d immunohistochemical staining of Sox2, Nanog, p-JAK2, p-STAT3 positive expression in GC and adjacent normal tissues (×400); *p < 0.05 vs. the injection of pcDNA3.1-transfected cells; ^#p < 0.05 vs. the treatment of pcDNA3.1-BMX-ARHGAP and PBS. Enumeration data in a were expressed as mean ± deviation and analyzed by one-way and repeated measurement analysis of variance; measurement data in b, c were analyzed by one-way analysis of variance; each experiment was repeated three times. BMX bone marrow kinase on chromosome X, ARHGAP RhoA GTPase activating protein, PBS phosphate buffer saline, JAK2 Janus kinase 2, STAT3 signal transducer and activator of transcription 3