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Fig. 4 | Cancer Cell International

Fig. 4

From: UBN2 promotes tumor progression via the Ras/MAPK pathway and predicts poor prognosis in colorectal cancer

Fig. 4

UBN2 inhibition reduces CRC cell migration and invasion and tumor metastasis in vitro and in vivo. a Cell migration was determined by using wound-healing assays. Histograms represent the percentage of wound-healing area in 72 h. b Quantification of the numbers of migrated cells by transwell migration assay. Histograms represent the number of invasive cells. c Three-dimensional morphology assay. Histograms represent the average number of filopodia formed by each cell sphere. d F-actin expression and pseudopodia were measured in UBN2-depleted SW837 and LoVo cells. e Primary tumors in the intestines formed in mice orthotopically implanted with SW837/shUBN2 and control SW837 cells. H&E staining and IHC staining by using antibodies against UBN2 and Ki-67 were shown. Scale bars: 50 µm. Histograms represent the percentage of Ki-67 positive cells. f Representative images of gross specimens and H&E staining of liver and vascular metastatic lesions. Scale bars: 50 µm. Error bars represent mean ± SD from three independent experiments. **p < 0.01

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