Fig. 5

ER stress was stimulated by BP5 in HCT116 cells. a, b Western blot analysis of ER stress–associated proteins. HCT116 cells were treated with 8 mM BP5 for the indicated durations (3, 6, 12 and 24 h). Then, the cell total proteins were obtained for Western blotting. One of three representative experiments is presented. The data shown are the mean ± SD (n = 3) (*p < 0.5, **p < 0.01, ***p < 0.001 vs. control). c–e Knockdown IRE1, ATF-6, and PERK regulates oncogenic phenotypes of HCT116 cells. HCT116 cells were transfected with specific siRNAs targeting IRE1, ATF-6, and PERK, or non-targeting siRNA (si-NC), and treated with BP5 (8 mM) for 24 h. Then, western blotting analysis were used for detection the levels of IRE-1, ATF6 and PERK expressions, CCK-8 assays were performed to evaluate the cell viabilities and flow cytometry was used to detect the apoptosis (*P < 0.05, ***P < 0.001 vs. BP5-treated group)