Fig. 6

Levels of intracellular ROS and cytosolic Ca²⁺ were elevated by BP5 in HCT116 cells. HCT116 cells were cultured with various doses of BP5 (2, 4 and 8 mM) for 24 h. a ROS production were quantified by using the H₂DCF-DA green fluorescence probe. Image observation were carried out by a fluorescence microscopy under ×40 magnification (scale bar, 0.1 mm). b Using ImageJ software, the mean fluorescence intensities of DCF were quantified (**p < 0.01, ***p < 0.001 vs. control). c The levels of cytosolic Ca²⁺ were quantified using Fluo-3 AM fluorescence probe. d The quantitative data of the levels of cytosolic Ca²⁺ shown are mean ± SD (n = 3) (*p < 0.5, **p < 0.01 vs. control)