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Fig. 7 | Cancer Cell International

Fig. 7

From: Bursopentin (BP5) induces G1 phase cell cycle arrest and endoplasmic reticulum stress/mitochondria-mediated caspase-dependent apoptosis in human colon cancer HCT116 cells

Fig. 7

Mitochondria-mediated apoptosis was stimulated by BP5 in HCT116 cells. Cells were cultured with BP5 (0, 2, 4 and 8 mM) for 24 h. a Flow cytometry analysis showed the distribution of JC-1 green positive cells with lower ΔΨm (lower right quadrant). b Quantitative analysis of the ratio of red to green fluorescence. c Western blot analysis was applied to quantify the levels of Bax, Bcl-2, Mito Cyt C, Cyto Cyt C, caspase-9 and -3 as well as cleaved caspase-3. d Densitometry analysis of Bax, Bcl-2, Mito Cyt C, Cyto Cyt C, caspase-9 and -3 as well as cleaved caspase-3. e Quantitative analysis of the ratio of cleaved caspase-3 and caspase-3. The quantitative data shown are mean ± SD (n = 3) (*p < 0.5, **p < 0.01, ***p < 0.001)

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Cancer Cell International

ISSN: 1475-2867

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