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Fig. 2 | Cancer Cell International

Fig. 2

From: Decreased SATB1 expression promotes AML cell proliferation through NF-κB activation

Fig. 2

SATB1 knockdown led to a progressive increase in the proliferation of HL-60 cells and accelerated S phase entry, but did not increase the invasiveness of AML cells. a SATB1 mRNA expression was decreased in HL-60 SATB1-shRNA1 and SATB1-shRNA2 cells (P = 0.0286, P = 0.0017). b, c Decreased SATB1 protein levels were confirmed by western blotting (P < 0.0001, P = 0.0002). d CCK assay was performed and the absorbance at 450 nm was measured. Increased proliferation of HL-60 cells was detected from day 2 to day 5. e Knockdown of SATB1 Cell cycle was analyzed by flow cytometry in SATB1-shRNA1, 2 and HL-60 CTR group. f The percentage of cells in SATB1-shRNA1/2 was reduced in the G0/G1 phase but increased in S phase (P = 0.0045, P < 0.0001). g Cell Invasion was analyzed by transwell assay and the result showed that down regulation of SATB1 did not increase the penetration of AML cells through the matrigel-coated membrane (P = 0.2134)

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