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Fig. 4 | Cancer Cell International

Fig. 4

From: Overexpression of lncRNA PIK3CD-AS1 promotes expression of LATS1 by competitive binding with microRNA-566 to inhibit the growth, invasion and metastasis of hepatocellular carcinoma cells

Fig. 4

Effect of overexpression of PIK3CD-AS1 on EMT of hepatocellular carcinoma cells. a Observation of cell morphology by an inverted microscope (×200); b detection of ZEB1 and Snail mRNA expression in cells by RT-qPCR; c detection of E-cadherin and Vimentin protein expression in cells by immunofluorescence staining (×400); d detection of E-cadherin and Vimentin protein expression by western blot analysis; the experiment was repeated three times; the data was analyzed by the t test or one-way ANOVA, and after ANOVA, the pairwise comparison was analyzed by the LSD-t test; *P < 0.05 vs the blank group

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