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Fig. 6 | Cancer Cell International

Fig. 6

From: Overexpression of lncRNA PIK3CD-AS1 promotes expression of LATS1 by competitive binding with microRNA-566 to inhibit the growth, invasion and metastasis of hepatocellular carcinoma cells

Fig. 6

The combination of PIK3CD-AS1 and miR-566. a Detection of miR-566 expression in HCC tissues and adjacent normal tissues by RT-qPCR, n = 68; b detection of miR-566 expression in HCC cells and normal liver cells by RT-qPCR; c detection of miR-566 expression in HCC cells by RT-qPCR; d bioinformatics websites predict binding sites for PIK3CD-AS1 and miR-566; e dual luciferase reporter gene assay to verify the regulatory relationship between PIK3CD-AS1 and miR-566; f the binding relationship between PIK3CD-AS1 and miR-566 in HCC cells by RNA-pull down; the experiment was repeated three times; the data was analyzed by the t test or one-way ANOVA, and after ANOVA, the pairwise comparison was analyzed by the LSD-t test; *P < 0.05 vs L-O2 cells or the blank group

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Cancer Cell International

ISSN: 1475-2867

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