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Fig. 1 | Cancer Cell International

Fig. 1

From: Mst1 overexpression combined with Yap knockdown augments thyroid carcinoma apoptosis via promoting MIEF1-related mitochondrial fission and activating the JNK pathway

Fig. 1

Mst1 overexpression and Yap knockdown induce the apoptosis of MDAT-32 cells. ac Proteins were isolated from MDAT-32 cells and then western blot was used to evaluate the content of Mst1 and Yap. Adenovirus Mst1 (ad-Mst1) and shRNA against Yap (sh-Yap) were transfected into MDAT-32 cells. d MTT assay was used to evaluate the cell viability of MDAT-32 cells. Adenovirus Mst1 (ad-Mst1) and shRNA against Yap (sh-Yap) were transfected into MDAT-32 cells. e LDH release assay was used to evaluate the death of MDAT-32 cells. f, g TUNEL staining was used to quantify the apoptosis of MDAT-32 cells. The number of TUNEL-positive cells was recorded. h ELISA assay was used to measure the activity of caspase-3 in response to Mst1 overexpression and/or Yap knockdown. *p < 0.05 vs. control group, #p < 0.05 vs. ad-Mst1 group and/or sh-Yap group

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