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Fig. 2 | Cancer Cell International

Fig. 2

From: Mst1 overexpression combined with Yap knockdown augments thyroid carcinoma apoptosis via promoting MIEF1-related mitochondrial fission and activating the JNK pathway

Fig. 2

Cell proliferation and migration are also modulated by Mst1 and Yap. ac Proteins were isolated from MDAT-32 cells and then western blot was used to evaluate the expression of Cyclin D1 and CDK4. Adenovirus Mst1 (ad-Mst1) and shRNA against Yap (sh-Yap) were transfected into MDAT-32 cells. d, e EdU staining was used to quantify the number of proliferated cells in response to Mst1 overexpression and/or Yap knockdown. g, h Transwell assay was used to evaluate the number of migrated cells in response to Mst1 overexpression and/or Yap knockdown. ig RNA was isolated from MDAT-32 cells and then qPCR was used to evaluate the transcription of Rac1 and ROCK1. Adenovirus Mst1 (ad-Mst1) and shRNA against Yap (sh-Yap) were transfected into MDAT-32 cells. *p < 0.05 vs. control group, #p < 0.05 vs. ad-Mst1 group and/or sh-Yap group

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