Fig. 3

Mst1 overexpression and Yap knockdown mediate mitochondrial dysfunction in MDAT-32 cells. a, b JC-1 probe was used to measure the mitochondrial membrane potential. Red fluorescence indicates the normal mitochondrial potential whereas green fluorescence means the damaged mitochondrial potential. c mPTP opening rate was measured in response to Mst1 overexpression and/or Yap knockdown. d, e Immunofluorescence assay was used to observe the translocation of cyt-c into nucleus. The expression of nuclear cyt-c was determined. f, g ROS production was measured via flow cytometry. Mst1 (ad-Mst1) and shRNA against Yap (sh-Yap) were transfected into MDAT-32 cells. h–l Proteins were isolated from MDAT-32 cells and then western blot was used to evaluate the levels of mitochondrial apoptosis proteins. Adenovirus Mst1 (ad-Mst1) and shRNA against Yap (sh-Yap) were transfected into MDAT-32 cells. *p < 0.05 vs. control group, ^#p < 0.05 vs. ad-Mst1 group and/or sh-Yap group