Fig. 5

Mst1 overexpression and Yap knockdown control mitochondrial fission via the JNK-MIEF1 pathway. a–c Proteins were isolated from MDAT-32 cells and then western blot was used to evaluate the levels of p-JNK and MIEF1. Adenovirus Mst1 (ad-Mst1) and shRNA against Yap (sh-Yap) were transfected into MDAT-32 cells. To prevent the activation of p-JNK, SP600125 was used. d, e Co-immunofluorescence assay for mitochondria and MIEF1. The length of mitochondria was measured and the fluorescence intensity was determined. Adenovirus Mst1 (ad-Mst1) and shRNA against Yap (sh-Yap) were transfected into MDAT-32 cells. To prevent the activation of p-JNK, SP600125 was used. *p < 0.05 vs. control group, ^#p < 0.05 vs. ad-Mst1 group and/or sh-Yap group