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Fig. 6 | Cancer Cell International

Fig. 6

From: Mst1 overexpression combined with Yap knockdown augments thyroid carcinoma apoptosis via promoting MIEF1-related mitochondrial fission and activating the JNK pathway

Fig. 6

JNK-MIEF1 pathway is also involved in Mst1/Yap-modulated cell viability and mitochondrial damage. a ATP production was determined to reflect the mitochondrial energy metabolism. Adenovirus Mst1 (ad-Mst1) and shRNA against Yap (sh-Yap) were transfected into MDAT-32 cells. To prevent the activation of p-JNK, SP600125 was used. b, c Immunofluorescence assay was used to observe the translocation of cyt-c into nucleus. The expression of nuclear cyt-c was determined. Adenovirus Mst1 (ad-Mst1) and shRNA against Yap (sh-Yap) were transfected into MDAT-32 cells. To prevent the activation of p-JNK, SP600125 was used. df The levels of cellular anti-oxidants were measured via ELISA. g MTT assay was used to observe the alterations of cellular viability. h LDH release assay was used to evaluate the cell death in response to Mst1 overexpression, Yap knockdown and JNK inactivation. *p < 0.05 vs. control group, #p < 0.05 vs. ad-Mst1 group and/or sh-Yap group

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