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Fig. 5 | Cancer Cell International

Fig. 5

From: The ionophore antibiotic gramicidin A inhibits pancreatic cancer stem cells associated with CD47 down-regulation

Fig. 5

Effects of GrA on the expression and distribution of pancreatic CSC markers. a Protein expression profiles of CD133 and CD44 in BxPC-3 and MIA PaCa-2 cells treated with 0.1, 0.01 and 0.001 μM of GrA for 48 h represented graphically. b Densitometry quantitation of CD44 band intensities in western blot. Cells were treated with GrA for 24 h and 48 h, respectively. c Immunofluorescence staining images showed CD47 distribution in BxPC-3 cells treated with 0.05 μM GrA for 24 h. d Immunofluorescence staining images showed CD47 distribution in MIA PaCa-2 cells treated with 0.05 μM GrA for 24 h. Cellular nucleus was stained by DAPI exhibiting blue fluorescence while CD47 on cell surface was marked by antibodies with green fluorescence. The white arrow indicates the CD47 cluster

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Cancer Cell International

ISSN: 1475-2867

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