Fig. 6

Effects of GrA and GEM combination. a The CDI values for GrA and GEM of BxPC-3 and MIA PaCa-2 cells were shown graphically. Viability of cultured MIA PaCa-2 cells treated with appropriate concentrations of GrA, GEM, or their combination for 48 h was determined by CCK-8 assay. Data of each concentration were from 3 replicated wells. b Western blot analysis of proteins from MIA PaCa-2 cells treated with 0.05 μM GrA, 0.05 μM GEM, or their combination for 3 h, 6 h, and 24 h, respectively. c Densitometry quantitation of 24 h treatment protein bands intensity of b. d Densitometry quantitation of VDAC band intensity from western blot experiment. Protein samples were obtained from MIA PaCa-2 cells treated with 0.05 μM, 0.005 μM GrA, GEM or their combination. *P < 0.05 in GrA + GEM 0.05 μM vs control and GrA + GEM 0.05 μM vs GEM 0.05 μM. e Western blot analysis of SIRPα and CD68 from RAW264.7 cells and THP-1 cells treated with 0.1 and 0.01 μM GrA