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Fig. 1 | Cancer Cell International

Fig. 1

From: Alpha-mangostin induces endoplasmic reticulum stress and autophagy which count against fatty acid synthase inhibition mediated apoptosis in human breast cancer cells

Fig. 1

α-Mangostin stimulated autophagy in MBA-MB-231 cells. a MDA-MB-231 cells were treated with 0, 1, 2, and 4 μM α-mangostin for 24 h, then strained by MDC. b MDA-MB-231 cells were treated with 0, 1, 2, and μM α-mangostin for 24 h, and then the relative expression levels of LC3II/LC31 and P62 were analyzed by western blot and were quantified densitometrically with the software ImageJ and calculated according to the reference bands of GAPDH. Data represented the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01. c Cells were treated with/without 4 μm α-mangostin followed 24 h incubation with/without 3MA and the relative expression levels of proteins were analyzed and quantified. Data represented the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01. d MDA-MB-231 cells were treated with/without 4 μm α-mangostin with/without transfected with siRNA targeting P62 for 24 h. Cell viabilities were then determined by the CCK-8 assay. Data represented the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01. α-M: α-mangostin

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