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Fig. 4 | Cancer Cell International

Fig. 4

From: Alpha-mangostin induces endoplasmic reticulum stress and autophagy which count against fatty acid synthase inhibition mediated apoptosis in human breast cancer cells

Fig. 4

The effects of ER stress inhibitor on autophagy, cell viability and apoptosis. a The relative expression levels of LC3II/LC3I and P62 in cells treated with/without 4 μm α-mangostin followed 24 h incubation with/without 4PBA were analyzed by western blot and quantified. Data represented the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01. b Cell were treated with/without 4 μm α-mangostin followed 24 h incubation with/without 4PBA. Cell viabilities were then determined by the CCK-8 assay. Data represented the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01. c Cells were treated with/without 4 μm α-mangostin followed 24 h incubation with/without 4PBA and double-stained with annexin V and PI and analyzed by flow cytometry. The gate setting distinguished between living (bottom left), necrotic (top left), early apoptotic (bottom right), and late apoptotic (top right) cells. d The percentage of apoptotic cells in each well was counted under flow cytometry. Data represented the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01

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Cancer Cell International

ISSN: 1475-2867

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