Fig. 8

Migration and invasion of HT29 cells were promoted by JAG2-rich exosomes. a HT29 cells were incubated with control exosomes or JAG2-rich exosomes (40 μg/mL) for 24 h. Cells were then fixed and stained. Subcellular localization of JAG2 in HT29 cells was changed when treated with JAG2-rich exosomes; b, c migration and invasion of the HT29 cells were promoted when treated with JAG2-rich exosomes; d, e relative expression levels of related mRNAs and proteins were determined in JAG2-rich exosome-treated HT29 cells by real-time quantitative PCR and Western blot, respectively. Data are the mean ± SD. of three independent experiments. *P < 0.05 and **P < 0.01 versus control