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Fig. 2 | Cancer Cell International

Fig. 2

From: The lncRNA TDRG1 promotes cell proliferation, migration and invasion by targeting miR-326 to regulate MAPK1 expression in cervical cancer

Fig. 2

Knockdown of TDRG1 suppressed cell proliferation in Hela and SIHA cells. a The expression levels of TDRG1 were examined by qRT-PCR in Hela and SIHA cells transfected with siRNAs targeted TDRG1 (siTDRG1#1, siTDRG1#2, siTDRG1#3). b Growth curves were analyzed by MTT assay in Hela and SIHA cells transfection with siTDRG1 and siNC for 0, 12, 48 and 72 h. c The effect of TDRG1 silencing on Hela and SIHA cell proliferation were determined by colony formation assay. d The effect of down-regulated TDRG1 on the cell cycle of Hela and SIHA cells were analyzed by flow cytometry assay. The cell cycle distribution was exhibited. e The influence of siTDRG1 on the protein levels including Cyclin D1, CDK4, CDK6, Cyclin E1, p-Rb and Rb were detected by western blot in Hela and SIHA cells. f The effect of silencing of TDRG1 on the apoptotic proteins-Bcl-2, BAX, cleaved Caspase 3, cleaved Caspase 9, cleaved PARP and PARP were checked by western blot in Hela and SIHA cell lines. Data were expressed as mean ± SD. **P < 0.01, ***P < 0.001

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