Fig. 4

TDRG1 inversely interacted with miR-326. a TDRG1 mRNA wide-type (TDRG1 WT) and the mutated-type (TDRG1 MUT) in the miR-326 binding sites were shown. Luciferase activity of HEK293T cells co-transfected with miR-326 mimics or NC mimics and luciferase reporters containing TDRG1 WT or TDRG1 MUT transcript were determined by dual-luciferase reporter assays. b Endogenous miR-326 precipitated by AGO2 upon overexpression of TDRG1 was determined by RIP assay in HEK293 cells. c The interaction of TDRG1 and miR-326 in HEK293 cells was detected by RNA pull-down assay which precipitated with bio-miR-326, bio-miR-326 Mut or bio-NC, and then the expression of TDRG1was checked by qRT-PCR. d The expression of miR-326 in CC tissues and corresponding normal tissues were detected by qRT-PCR. n = 30. e The correlation between TDRG1 and miR-326 was analyzed. Data were expressed as mean ± SD. **P < 0.01, ***P < 0.001