Fig. 5

MAPK1 was directly targeted by miR-326 and positively regulated by TDRG1. a MAPK1 3′-UTR wide-type (MAPK1 3′-UTR WT) and the mutated-type (MAPK1 3′-UTR MUT) in the miR-326 binding sites were shown. Luciferase activitis of HEK293 cells co-transfected with miR-326 mimics or NC mimics and luciferase reporters containing MAPK1 3′-UTR WT or MAPK1 3′-UTR MUT transcript were determined by dual-luciferase reporter assays. b, c The levels of MAPK1 in Hela and SIHA cells transfected with miR-326 mimics or NC mimics, as well as miR-326 inhibitor or NC inhibitor were analyzed by qRT-PCR and western blot. d, e The expression levels of TDRG1 were detected in Hela and SIHA cells treated with TDRG1 overexpression or control plasmid using qRT-PCR assay. The levels of MAPK1 in Hela and SIHA cells transfected with TDRG1 overexpression or control plasmid, as well as siTDRG1 or siNC were analyzed by qRT-PCR and western blot. f The expression of MAPK1 in CC tissues and corresponding normal tissues were detected by qRT-PCR. n = 30. g The correlation between MAPK1 and TDRG1, as well as MAPK1 and miR-326 expression was analyzed. h The protein level of MAPK1 in CC patients and normal subjects were measured by western blot. n = 3. Data were expressed as mean ± SD. *P < 0.05, ***P < 0.001 r