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Fig. 2 | Cancer Cell International

Fig. 2

From: β-Asarone increases doxorubicin sensitivity by suppressing NF-κB signaling and abolishes doxorubicin-induced enrichment of stem-like population by destabilizing Bmi1

Fig. 2

β-Asarone suppresses stem-like properties. a, b Raji cells were treated with the indicated concentration of β-Asarone for 72 h. ALDH activity was determined by ALDEFLUOR assay and flow cytometry analysis. Representative results were shown in a and statistical results were shown in b. c Raji cells were treated with the indicated concentration of β-Asarone for 72 h. Cells were subjected to western blot analysis. dg Raji cells were grown under sphere culture condition for 3 days. Then, the spheres were divided equally into two parts. One was treated with β-Asarone (20 μM) for another 3 days. The other was treated with a control solvent for another 3 days. The spheres were photographed and the diameters of the spheres were calculated. The representative images were shown in d. The size distribution of spheres was shown in e. Spheres with a diameter larger than 60 μm were counted and shown in f. The expression of cancer stem cell-related proteins was determined by western blot (g). hk β-Asarone was washed out from the sphere. Raji cells were detached from sphere to perform sphere passage assay. The representative images were shown in h. The size distribution of spheres was shown in i. Spheres with a diameter larger than 60 μm were shown in j. The expression of cancer stem cell-related proteins was determined by western blot (k). l The Raji cells used to perform sphere formation assay and sphere passage assay were also grown under its regular culture medium for 3 days to evaluate proliferation using CCK8 assay. The bar represents mean ± SD of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, the ANOVA test, followed by Least Significant Difference test, were used to make statistical comparisons). The horizontal line within each box in e and i represented the median value. The Kruskal–Wallis test, followed by Dunn’s Multiple Comparison test, were used to make statistical comparisons (***p < 0.001)

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