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Fig. 3 | Cancer Cell International

Fig. 3

From: β-Asarone increases doxorubicin sensitivity by suppressing NF-κB signaling and abolishes doxorubicin-induced enrichment of stem-like population by destabilizing Bmi1

Fig. 3

Synergistic effects of β-Asarone and doxorubicin in Raji lymphoma cells. a Raji cells were treated with various concentrations of drugs alone or in combination for 72 h. β-Asarone and doxorubicin were combined in a fixed ratio (1:1). The highest concentrations of either drug was 100 μM, the lower concentrations of either drug were 1/2 of previous higher concentrations of either drug. The growth inhibitory effects were determined by an CCK8 assay. b The combination indices (CI) were calculated from data obtained in a. The plots showed the fraction of Raji cells that were affected by the β-Asarone and doxorubicin combinations. cg Raji cells were treated with the indicated concentration of β-Asarone or (and) doxorubicin for 72 h. Apoptosis was evaluated by the Annexin V-FITC/PI staining and flow cytometry analysis. Representative results were shown in c and statistical results were shown in d. The expression of apoptotic proteins was determined by western blot (e). The representative flow cytometric histogram (f) of intracellular uptake of doxorubicin and the statistic result were shown in g. Bar represents mean ± SD of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, the ANOVA test, followed by Least Significant Difference test, were used to make statistical comparisons)

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