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Fig. 6 | Cancer Cell International

Fig. 6

From: β-Asarone increases doxorubicin sensitivity by suppressing NF-κB signaling and abolishes doxorubicin-induced enrichment of stem-like population by destabilizing Bmi1

Fig. 6

β-Asarone promotes proteasomal degradation of Bmi1 to reduce the stem-like population. a, b Bmi1 was expressed in Raji cells via lentivirus vector. Cells expressed Bmi1 and control cells were treated with or without the indicated concentration of β-Asarone. Western blot (a) or ALDEFLUOR assay (b) was performed at the indicated time points. Bars represent mean ± SD of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, the ANOVA test, followed by Least Significant Difference test, were used to make statistical comparisons). c Raji cells were treated with or without the indicated concentration of β-Asarone. PCR examined Bim1 and GAPDH expression. d, e Cells were treated with CHX for the indicated time points and western blot analysis of Bmi1 and GAPDH was performed (d). The densitometric quantification of Bmi1 normalized to GAPDH was plotted against various time points to determine its half-life (e). f Cells were treated with MG132 (10 μM) or lysosomal inhibitor Chloroquine (25 μM) as indicated. Cells were subjected to western blot analysis. g Cells were treated with the indicated concentration of β-Asarone for 72 h and PCR analysis of p16INK4a, p14ARF and GAPDH was performed

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