Fig. 2

NPL4 plays an essential role in BC cell proliferation. a T24 cells were transfected with si-NPL41, si-NPL4-2, or negative control (si-NC). Western blot analysis and qRT-PCR were used to examine NPL4 expression. Bottom panel shows densitometric analysis of three independent experiments. ***P < 0.001 vs. si-NC. b T24 cells were transfected with NPL4 overexpression vector pcDNA3.1–NPL4 or empty vector. Western blot analysis and qRT-PCR were used to examine NPL4 expression. Bottom panel shows densitometric analysis. **P < 0.05, ***P < 0.01 vs. empty vector. c, d T24 cells were prepared as in a and b, and MTT and colony formation assays were used to detect the cell viability. Right panel shows colony number analysis. ***P < 0.01 vs. corresponding control