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Fig. 4 | Cancer Cell International

Fig. 4

From: Upregulation of NPL4 promotes bladder cancer cell proliferation by inhibiting DXO destabilization of cyclin D1 mRNA

Fig. 4

NPL4 interacts with DXO and negatively regulates DXO protein expression. a GeneMANIA used to analyzed network of proteins according to the databases. All the nodes were connected and related to NPL4. b T24 cells were transfected with si-NPL4 (or si-con) and pcDNA3.1-NPL4 (or empty vector). Western blot analysis was used to detect DXO protein levels. c T24 cells were transfected with NPL4 overexpression pcDNA–3.1NPL4 or empty vector. CoIP analysis was used to detect the interaction between DXO and NPL4. d T24 cell were treated with MG132 or DMSO for 6 h and then transfected with NPL4 overexpression pCDNA-3.1NPL4 or empty vector. DXO protein level was detected by western blot analysis. e The relationships between NPL4 and DXO was analyzed by Pearson correlation.(R = − 0.3639, P = 0.0406)

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