Skip to main content
Fig. 4 | Cancer Cell International

Fig. 4

From: MiR-503 suppresses cell proliferation and invasion of gastric cancer by targeting HMGA2 and inactivating WNT signaling pathway

Fig. 4

HMGA2 is a target of miR-503 in GC cells. a Base-pairing interaction between miR-503 seed sequences and HMGA2, as predicted by TargetScan. The wild-type (WT) HMGA2-3′UTR containing the putative miR-503 binding site or mutant HMGA2-3′UTR (MUT) (from GCUGCU to CGACGA) was cloned into the SpeI and MluI sites of the pMIR-Report luciferase vectors. b The luciferase activity was shown when MKN45 cells were cotransfected with pMIR-HMGA2-3′UTR-WT or pMIR-HMGA2-3′UTR-MUT and miR-503 mimic or miR-NC oligonucleotides. c, d The mRNA expression levels of HMGA2 was detected using qRT-PCR analysis after cell transfected with miR-NC, miR-503 mimic or miR-503 inhibitor in MKN-45 or SGC-7901 cells. e, f The protein expression levels of HMGA2 was detected using qRT-PCR analysis after cell transfected with miR-NC, miR-503 mimic or miR-503 inhibitor in MKN-45 or SGC-7901 cells. All values are presented as mean ± SD, *P < 0.05

Back to article page