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Fig. 3 | Cancer Cell International

Fig. 3

From: Vitamin E δ-tocotrienol sensitizes human pancreatic cancer cells to TRAIL-induced apoptosis through proteasome-mediated down-regulation of c-FLIPs

Fig. 3

The effects of TRAIL and VEDT on cell survival. a Effect of TRAIL alone (25 and 50 ng/mL) or in combination with VEDT at multiple concentrations (10, 25, 50 μM) on cell survival at 24 h in panel of human pancreatic cancer cell lines. Columns, means; bars, standard deviation (n = 3, *P < .05 and **P < .01). b Effect of TRAIL alone or in combination with VEDT on the enzymatic activity of caspase-3 in panel of human pancreatic cancer cell lines after 24 h. Columns, means; bars, standard deviation (n = 3, *P < .05 and **P < .01). c Western blot analysis of cleavage of caspase-8, caspase-3, and PARP (CF = cleaved fragment) in a panel of human pancreatic cancer cell lines following treatment with TRAIL alone or in combination with VEDT for 24 h (n = 3). β-Actin served as loading control. d Flow cytometry analysis of apoptosis as measured by annexin V staining following treatment with TRAIL alone (25 ng/mL) or in combination with VEDT (50 μM) in MiaPaCa-2 cells (n = 3). In annexin V assay, percentages of positive cells in the top right and bottom right quadrants were added to get the total apoptotic cells. *P < .001 compared with untreated cells; **P < .01 compared with untreated cells

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