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Fig. 6 | Cancer Cell International

Fig. 6

From: Vitamin E δ-tocotrienol sensitizes human pancreatic cancer cells to TRAIL-induced apoptosis through proteasome-mediated down-regulation of c-FLIPs

Fig. 6

The effect of VEDT on c-FLIP. a Effect of VEDT treatment on c-FLIP messenger RNA levels in MiaPaCa-2 and BxPc-3 cells at 6 h. 18S rRNA served as internal control for each sample (n = 3). b Half-life of c-FLIPs protein in MiaPaCa-2 cells as determined by cyclohexamide (CHX) (25 µg/ml) pretreatment for the indicated duration, following 12-hour treatment with either vehicle (ethanol) or VEDT (n = 3). c Effect of MG132 protease inhibitor on c-FLIP protein levels in presence or absence of VEDT in a panel of pancreatic cancer cell lines (MiaPaCa-2, SW1990, BxPc-3 and Panc-1). The cells were pretreated with MG132 (25 µM) for 6 h followed by VEDT treatment for 24 h (n = 3). d Effect of MG132 protease inhibitor on ubiquitin-bound c-FLIP in the presence or absence of VEDT in MiaPaCa-2 cells. The cells were pretreated with MG132 (25 µM) for 6 h followed by VEDT treatment for 24 h (n = 3)

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