Skip to main content
Fig. 3 | Cancer Cell International

Fig. 3

From: DNA damage-induced activation of ATM promotes β-TRCP-mediated ARID1A ubiquitination and destruction in gastric cancer cells

Fig. 3

A canonical DSGXXS in ARID1A mediated the interaction between β-TrCP and ARID1A. a Alignment of amino acids corresponding to the DSGxxS sequence with ARID1A orthologs and other β-TrCP substrates. b HEK293T cells were co-transfected with HA-β-TrCP and Flag-ARID1A WT or Flag-ARID1A SA mutant for 36 h, cell extracts were IP with anti-HA beads, and the immunocomplexes were subjected to western blot with the indicated antibodies. c NCI-N87 cells were transfected Flag-ARID1A WT or Flag-ARID1A SA or SE mutants for 24 h, and then treated with or without 2 μg/ml VP16 for additional 12 h. Cell extracts were subjected to western blot with the indicated antibodies. d NCI-N87 cells were transfected with HA-β-TrCP, his-ubiquitin with Flag-ARID1A WT or MUT plasmids for 24 h, and then treated with 2 μg/ml VP16 for additional 6 h, 20 μM MG132 was added 4 h before cell harvest. Cell extracts were purified with Ni+ beads, and the immunocomplexes were subjected to western blot with the indicated antibodies

Back to article page