Fig. 4

ATM-induced ARID1A phosphorylation promoted β-TrCP-induced ARID1A destruction. a NCI-N87 cells were co-transfected with Flag-ARID1A and HA-β-TrCP for 36 h, 20 μM MG132 was added for additional 6 h. Cell extracts were treated with λ-PPase for 1 h at 30 °C and IP with anti-HA beads. The immunocomplexes were subjected to western blot with the indicated antibodies. b NCI-N87 cells were transfected with con-shRNA or shRNA specific against ATM for 24 h and then treated with or without 2 μg/ml VP16 for additional 6 h, cell extracts were IP with anti-Flag M2 beads, and the immunocomplexes were subjected to western blot with the indicated antibodies. c NCI-N87 cells were transfected with con-shRNA or shRNA specific against ATM or ATR for 24 h and then treated with or without 2 μg/ml VP16 for additional 6 h, 25 μg/ml (cycloheximide) CHX was added for the indicated time course. Cell extracts were subjected to western blot with the indicated antibodies