Fig. 9

Harmine could suppress Muv phenotype on some components of the LET-60 signaling pathway. a and b Harmine inhibits the Muv phenotype in kuIs57, a strain that overexpresses the lin-45 hyperactivation mutation LIN-45-AA (S312A/S453A). c and d Harmine has no effect on let-23(sa62) mutants, with a gf mutation in EGFR. e and f Harmine moderately decreases the percentage of Muv animals and the number of ectopic vulvas in jgIs25, a transgenic strain overexpressing a chimeric protein consisting of the LET-23 N-terminus and the human EGFR C-terminus carrying the tyrosine kinase mutations T790M–L858R. White arrows indicate pseudovulvas and black arrowheads indicate normal vulvas. *p < 0.05; **p < 0.01; ***p < 0.001 by unpaired Student’s t-test