From: Effects of pericytes and colon cancer stem cells in the tumor microenvironment
miRNA | Expression level | Target | Findings | Technique | References |
---|---|---|---|---|---|
21* | ↑ | ITGβ4 | A prognostic tool, proliferation, invasion and metastasis | qPCR | |
23a | ↑ | E-cadherin | Induced EMT process associated CRC metastasis | qPCR | |
↓ | ZO-1 | Increasing vascular permeability and migration |  | ||
24* | ↑ | Paxillin | Inhibited the killing effect of NK cells to colorectal cancer cells | qRT-PCR and Western blot | |
34a | ↓ | Inh3 | Increased lymph node infiltration and metastasis in colon cancer patients | miRNA target prediction software miRWalk | |
126 | ↑ | BCL-2 and p53 | Potential tumour suppressor | qRT-PCR | |
137 | ↓ | TCF4 | Suppresses cell proliferation, migration and invasion in colon cancer cell lines | RT-qPCR | |
143* | ↑ | IGF-IR | Inhibited cell proliferation, migration, tumor growth, angiogenesis and increased chemosensitivity to oxaliplatin treatment | qRT-PCR and western blot | |
150 | ↑ | c-Myb | Inhibits cell proliferation, induces cell apoptosis and inhibits cell migration and invasion in human CRC cells | qRT-PCR, western blot and DNA constructs and luciferase target assay | |
200 | ↓ | ZEB1, ETS1 and FLT1 | Increased EMT | TaqMan MicroRNA assays | |
203 | ↑ | SOCS3 | Potential for metastasis; promoted the differentiation of monocytes to M2 macrophages | RT-qPCR and Gene Set Enrichment Analysis (GSEA) TargetScan and miRanda | |
221 | ↑ | RECK, RelA and STAT3 | Migration and invasion in vitro and metastasis in vivo | qRT-PCR and western blot qRT-PCR and western blot | |
1246 | ↑ | CCNG2 | Promoted the proliferation, colony formation, invasion and migration, and inhibited the apoptosis | RT-qPCR and Dual luciferase reporter assay |