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Fig. 3 | Cancer Cell International

Fig. 3

From: Monocarboxylate transporter 1 and monocarboxylate transporter 4 in cancer-endothelial co-culturing microenvironments promote proliferation, migration, and invasion of renal cancer cells

Fig. 3

Co-culturing promoted the invasion ability of 786-O cells in an MCT-dependent manner. A, B In the transwell chamber invasion assay, 786-O cells at a density of 1 × 104 cells/well were seeded in the upper chamber in serum-free medium supplemented with or without 10 µM 7ACC1, while 786-O cells or HUVECs at a density of 4 × 104 cells/well were seeded in the lower chamber in complete culture medium. 24 h after culturing, the 786-O cells that penetrated the membrane were counted under a microscope. A Representative images show the membrane-invaded 786-O cells (magnification of ×200). B Summarized results on the cell number of membrane-invaded 786-O cells in each culturing condition. For the control, the 786-O cells were seeded in both the upper and lower chambers; in the co-culture, 786-O cells were added to the upper chamber while HUVECs were added to the lower chamber. ***P < 0.001, between the indicated groups

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