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Fig. 2 | Cancer Cell International

Fig. 2

From: Long non-coding RNA MEG3 inhibits cervical cancer cell growth by promoting degradation of P-STAT3 protein via ubiquitination

Fig. 2

The mutual regulation between MEG3 and P-STAT3. The expression of P-STAT3 and MEG3 in cervical cancer tissues(n = 22)was detected by ELISA and RT-qPCR assays. Then Pearson correlation coefficient was calculated (a). RT-qPCR and western blot analysis results showed that the expression of P-STAT3 and c-MYC proteins decreased while that of caspase 3 and cleaved caspase 3 increased in Siha cells with high-level expression of MEG3. On the contrary, HeLa cells with low-level expression of MEG3 showed an increase in the expression of P-STAT3 and c-MYC proteins and a decrease in the expression of caspase 3 and cleaved caspase 3 (b, c). Immunofluorescence results confirmed that P-STAT3 protein was highly expressed in the cells from MEG3 group, while its expression was low in the cells from MEG3 shRNA group. Scale bar = 50 μm (d). RT-qPCR and luciferase assays showed that MEG3 had no significant effect on STAT3 gene expression (e). RT-qPCR and western blot analysis results revealed the absence of any significant change in the expression of MEG3 after upregulation or interference of STAT3 expression in cervical cancer cells (f, g). *P < 0.05

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