Fig. 1

Results of quantitative real time PCR (qRT-PCR). qRT-PCR was used to validate the results of small RNA-Sequencing. The qRT-PCR results demonstrated an increase in the expression of hsa-miR-10b-5p (6.50 ± 0.09-fold), hsa-miR-143-3p (2.70 ± 0.16-fold), and hsa-miR-145-5p (5.60 ± 0.22-fold) in the EEC tissues, whereas the expression of hsa-miR-369-3p (− 7.50 ± 0.24-fold), hsa-miR-136-3p (− 3.72 ± 0.14-fold), hsa-miR-451a (− 2.69 ± 0.13-fold), hsa-miR-493-5p (5.60 ± 0.21-fold), and hsa-miR-543 (− 4.51 ± 0.16-fold) decreased in the EEC tissues, respectively. These data were consistent with the small RNA-sequencing results. T, EEC tissues; C, their adjacent no cancerous counterparts. *p < 0.05