Skip to main content
Fig. 1 | Cancer Cell International

Fig. 1

From: YAP promotes multi-drug resistance and inhibits autophagy-related cell death in hepatocellular carcinoma via the RAC1-ROS-mTOR pathway

Fig. 1

YAP is critical for the multi-drug resistance of HCC cell lines. a The protein expression of YAP and main components of the Hippo pathway in the BEL/FU and BEL-7402 cell lines were analysed by western blot. β-Actin was used as a loading control. b The mRNA level of YAP in the BEL/FU and BEL-7402 cell lines. c The mRNA level of ABCC4, ABCC6, ABCB1 and ABCB4 in BEL/FU and BEL/7402 cells (left panel). The mRNA level of ABCC4, ABCC6, ABCB1 and ABCB4 in BEL/FU cells with or without YAP knockdown (right panel). d Nuclear and cytoplasmic YAP expression in the BEL/FU and BEL-7402 cell lines. NP: nuclear protein, CP: cytoplasmic protein. β-Actin or lamin A were used as loading controls. e, f The percentage of apoptotic BEL-7402 cells with or without YAP overexpression after treatment with PBS, 5-Fu (20 µg/ml) or DOX (0.5 µmol/ml) for 48 h was analysed by flow cytometry. g The amount of the apoptosis markers, cleaved PARP and cleaved caspase-3, in BEL-7402 cells with or without YAP overexpression after treatment with 5-Fu (20 µg/ml) or DOX (0.5 µmol/ml) for 48 h was analysed by western blot analysis. h The IC50 values of 5-Fu and DOX in BEL-7402 cells with or without YAP overexpression (indicated as YAP-OE and YAP-Ctrl, respectively) were analysed by CCK-8 assay. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ns, no significance

Back to article page