Fig. 4

YAP downregulated RAC1, resulting in an attenuation of ROS production. a, b Intracellular ROS were examined in BEL/FU cells after treatment with 5-Fu (0.3 mg/ml) for 48 h through flow cytometry using CellROX™ Reagent. NAC (0.75 mM) was used for ROS clearance. c The protein level of RAC1 was examined in BEL/FU cells with YAP knockdown or overexpression by western blot. d, e The expression of YAP, RAC1 and 8-OHdG was detected in the HCC tissue microarray (n = 120) by IHC (scale bar: 50 µm/25 µm). The YAP level was negatively correlated with the expression of RAC1 and 8-OHdG based on the IRS. f, g NAC abolished the growth inhibition of BEL/FU cells induced by the combined treatment of verteporfin (0.3 µg/ml) and 5-Fu (0.3 mg/ml) or DOX (2 µmol/ml). Data are presented as the mean ± SD. **p < 0.01