Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: Circadian protein BMAL1 promotes breast cancer cell invasion and metastasis by up-regulating matrix metalloproteinase9 expression

Fig. 2

The role of BMAL1 on MMP9 mRNA and protein levels along with the promoter activity of MMP9. a RT-PCR showing the effects of BMAL1 overexpression on the mRNA levels of MMP9 in ZR-75-30 cells. The bar graphs show the fold changes in relative mRNA levels normalized against GAPDH (right). b, c T47D and ZR-75-30 cells were transfected with HA-BMAL1 or control vector, and shBMAL1 or shControl. The samples (cells and medium) were subjected to western blot analysis with the indicated antibodies and zymography. d ZR-75-30 cells were transfected with pMMP9-luciferase and HA-BMAL1. Luciferase activity was measured and normalized against the b-galactosidase activity. For comparison, the pMMP9-Luc activity level of control cells was set to 1. All experiments were repeated at least three times. Data are presented as mean ± SD (p < 0.05, significant; **, p < 0.01; ***, p < 0.001)

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us