Fig. 3

The role of BMAL1 on NF-κB signaling pathway. a Real-time PCR showing the effects of BMAL1 overexpression on the mRNA expression levels of TNF-a, VEGFA, and uPA and IL8 in ZR-75-30 cells. All experiments were repeated at least three times. b NF-κB promoter reporter plasmid, and HA-BMAL1 or control vector were co-transfected into ZR-75-30 cells to determine luciferase activities. c Site-specific mutants or deletion for NF-kB (MMP9-del or NF-kB-mut), derived from the − 795/+ 19 construct of human MMP9 promoter reporter plasmids, and HA-BMAL1 or control vector were co-transfected into ZR-75-30 cells to determine luciferase activities. All experiments were repeated at least three times. Data are presented as mean ± SD (p < 0.05, significant; ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001)