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Fig. 5 | Cancer Cell International

Fig. 5

From: Circadian protein BMAL1 promotes breast cancer cell invasion and metastasis by up-regulating matrix metalloproteinase9 expression

Fig. 5

CBP is recruited by BMAL1 to transcriptionally regulate the acetylation of p65. a Western blot assay showed the effect of BMAL1 overexpression or knockdown on IκBα protein levels in ZR-75-30 cells. b The effect of the binding of p65 to IκBα was detected by immunoprecipitation with the indicated antibodies. c HEK 293T cells transfected with Myc-CBP only, or with HA-BMAL1 and Myc-CBP were subjected to immunoprecipitation with anti-Myc antibody followed by Western blot with anti-HA antibody or vice versa. d HEK 293T cells transfected with Myc-CBP only, or with GFP-p65 and Myc-CBP were subjected to immunoprecipitation with anti-Myc antibody followed by Western blot with anti-GFP antibody or vice versa. e HEK 293T cells transfected with control vector, GFP-p65 and Myc-CBP, or with GFP-p65, Myc-CBP and HA-BMAL1, were subjected to immunoprecipitation with anti-GFP antibody followed by Western blot with anti-HA and anti-Myc antibodies. f HEK 293T cells were transfected with the indicated plasmids for 24 h. The extract was immunoprecipitated with anti-GFP antibody and then probed with anti-acetylated lysine antibody. All experiments were repeated at least three times

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