Fig. 4

PHLDB2 knockdown attenuates TGF-β induced cell migration, invasion, and E-Cadherin reduction. a HCT116 p53^−/− cells were transfected with non-specific control siRNA and siRNA against PHLDB2 at 40 nM. 24 h after transfection, the cells were replated into 6-well plates to attach for 6 h and the scratch was made for 0 h time point. Then the cells were treated with vehicle or 5 ng/ml TGF-β for another 24 h for imaging. b HCT116 p53^−/− cells were transfected with non-specific control siRNA and siRNA against PHLDB2 at 40 nM. 24 h after transfection, the cells were replated into Matrigel-coated upper champers (24-well transwell inserts) and allowed to attach for 6 h before vehicle or 5 ng/ml TGF-β were added to the lower wells for another 24 h and subjected to invasion assay. c Quantitation of 3 independent wound healing assays. d Quantitation of 3 independent invasion assays. e Cells mentioned in (a) and (b) were harvested in parallel for Western Blot analysis. *p < 0.05 as compared with vehicle control. **p < 0.05 as compared with TGF-β alone