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Fig. 6 | Cancer Cell International

Fig. 6

From: Interfering cellular lactate homeostasis overcomes Taxol resistance of breast cancer cells through the microRNA-124-mediated lactate transporter (MCT1) inhibition

Fig. 6

Re-sensitization of Taxol resistant cells by MCT1 inhibition. a MCF7 Taxol resistant cells were treated with control, Taxol alone, MCT1 inhibitor alone or the combination of Taxol and MCT1 inhibitor at the indicated concentrations for 48 h, the cell viabilities were measured. b MCF7 Taxol resistant cells were transfected with control miRNAs or miR-124 for 48 h, followed by the treatments of Taxol at 0, 10, 20, 40, 80 and 160 nM for 48 h. Cell viabilities were measured by MTT assay. c MCF Taxol resistant cells were treated with control, miR-124 or MCT1 inhibitor for 48 h, the glucose uptake, (d) cellular ATP/ADP ratio, (e) intracellular lactate and (f) extracellular lactate were measured. g MCF7 Taxol resistant cells were co-transfected with miR-124 plus control plasmid or miR-124 plus MCT1 overexpression plasmid for 48 h, the expressions of MCT1 were detected by Western blot and (H) the cell viabilities were measured under Taxol treatments. β-actin is a loading control. *p < 0.05; **p < 0.01

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