Fig. 2

miR-497-5p was upregulated in HCC cell lines and promoted the proliferation and migration of HCC cells. a The level of miR-497-5p in HCC and L02 cell lines. b Wound healing assay was performed to measure the effect of miR-NC (cells transfected with empty lentiviral vectors served as a negative control) and miR-inhibitor (cells transfected with lentiviral vectors with short hairpin RNA targeting miR-497-5p) on the migration of Huh7 and HepG2 cells. c CCK-8 assay after 72 h and 96 h of treatment with miR-inhibitor in Huh7 and HepG2 cells. d The invasion ability of Huh7 and HepG2 cells was measured by transwell assay (original magnification, ×200). e Western blot analysis showed that miR-497-5p promoted the expression of p-Rb, Cyclin D1, CDK4 and Cyclin E and inhibited the expression of P16. Error bars represent the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01. miR microRNA-497-5p