Fig. 5

XIST negatively regulated miR-497-5p in HCC. a Wild-type (WT) or mutated (MUT) XIST fragments containing the predicted miR-497-5p binding sites. b Luciferase activity assay revealed that the miR-497-5p mimic suppressed XIST-WT luciferase activity, while it had no effect on XIST-MUT luciferase activity in HCC cells. c The expression of XIST in 77 paired HCC tissues and their matched adjacent tissues was examined by qRT-PCR. d The correlation between XIST and miR-497-5p in HCC tissues. e The expression levels of XIST in Huh7 and HepG2 cells transfected with XIST-WT, XIST-MUT and negative control were examined using qRT-PCR. f The expression levels of miR-497-5p in Huh7 and HepG2 cells transfected with XIST-WT, XIST-MUT and negative control were determined by qRT-PCR. Error bars represent the mean ± SD from three independent experiments. *p < 0.05, ***p < 0.001. NC negative control, MUT mutated, WT wild type