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Fig. 2 | Cancer Cell International

Fig. 2

From: p53 sensitizes chemoresistant non-small cell lung cancer via elevation of reactive oxygen species and suppression of EGFR/PI3K/AKT signaling

Fig. 2

p53 is required for suppression of EGFR activity and CDDP-induced apoptosis in NSCLC cells. Chemoresistant H1299 cells were infected with adeno virus p53 (Adv-p53) (Multiplicity of Infection, MOI = 0, 0.25, 0.5 and 1; 5 h) followed by CDDP treatment (10 μM, 24 h). a Protein contents of P-EGFR (Try 1068), EGFR, P-p53 (Ser 15), p53 and GAPDH (loading control) were detected by Western blot. b Statistical analysis and quantification of indicated proteins [P-EGFR (Try 1068), EGFR, P-p53 (Ser 15) and p53] were performed. c Apoptosis was assessed by Hoechst staining. d A549 cells were transfected with either p53 siRNA or its scramble control (100 nM, 16 h) and treated with CDDP (10 µM, 24 h). Protein levels of p-EGFR, EGFR, P-p53, p53, GAPDH (loading control) were detected by Western blot. e Quantification and statistical analysis of indicated proteins were conducted. f Apoptosis before and after p53 knockdown (siRNA) and CDDP treatment were determined. All data were presented as mean ± SEM (n = 3). Data were analyzed by two-way ANOVA and Bonferroni post hoc test (*P < 0.05, **P < 0.01, ***P < 0.001; CTL vs. CDDP; #P < 0.05, #P < 0.01, ####P < 0.0001, Scramble vs. p53 siRNA)

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